Revised amino acid sequence of the B‐chain of ricin D due to loss of tryptophan in the cyanogen bromide cleavage
Authors:
- Tomohiro ArakiTATomohiro Araki
- Gunki FunatsuGFGunki Funatsu
- 21 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 191 (1) , 121-124
Abstract
The complete amino acid sequence of the ricin D B‐chain was redetermined using the DABITC/PITC double‐coupling method. By comparing the presently established amino add sequence with the sequence reported earlier which was determined by the manual Edman degradation technique, it was found that the identification of 26 amino acid residues had to be revised. Furthermore, the ricin D B‐chain was shown to consist of 262 amino acid residues rather than 260 as previously described. In addition, based on the new amino acid sequence, the sequence homology between the N‐ and C‐terminal domain of this chain was found to be 31%.Keywords
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