An Electrochemical Nanosensor for Monitoring the Dynamics of Intracellular H2O2 Upon NADH Treatment
Authors:
- 8 March 2023
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition
- Vol. 62 (16)
Abstract
Reactive oxygen species (ROS)‐based therapeutic strategies play an important role in cancer treatment. However, in situ, real‐time and quantitative analysis of intracellular ROS in cancer treatment for drug screening is still a challenge. Herein we report one selective hydrogen peroxide (H2O2) electrochemical nanosensor, which is prepared by electrodeposition of Prussian blue (PB) and polyethylenedioxythiophene (PEDOT) onto carbon fiber nanoelectrode. With the nanosensor, we find that the level of intracellular H2O2 increases with NADH treatment and that increase is dose‐dependent to the concentration of NADH. High‐dose of NADH (above 10 mM) can induce cell death and intratumoral injection of NADH is validated for inhibiting tumor growth in mice. This study highlights the potential of electrochemical nanosensor for tracking and understanding the role of H2O2 in screening new anticancer drug.Keywords
Funding Information
- National Natural Science Foundation of China (21874152 and 22174162)
This publication has 69 references indexed in Scilit:
- The NOX toolbox: validating the role of NADPH oxidases in physiology and diseaseCellular and Molecular Life Sciences, 2012
- Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach?Nature Reviews Drug Discovery, 2009
- How mitochondria produce reactive oxygen speciesBiochemical Journal, 2008
- NAD + /NADH and NADP + /NADPH in Cellular Functions and Cell Death: Regulation and Biological ConsequencesAntioxidants & Redox Signaling, 2008
- Nanoelectrochemistry of mammalian cellsProceedings of the National Academy of Sciences, 2008
- P2X7 receptors mediate NADH transport across the plasma membranes of astrocytesBiochemical and Biophysical Research Communications, 2007
- Hybrid bioelectrocatalyst for hydrogen peroxide reduction: Immobilization of enzyme within organic–inorganic film of structured Prussian Blue and PEDOTBioelectrochemistry, 2007
- A Reappraisal of Xanthine Dehydrogenase and Oxidase in Hypoxic Reperfusion Injury: the Role of NADH as an Electron DonorFree Radical Research, 1998
- MPP+ and MPDP+ induced oxygen radical formation with mitochondrial enzymesFree Radical Biology and Medicine, 1993
- NADH oxidase of plasma membranesJournal of Bioenergetics and Biomembranes, 1991