Protease-catalysed Direct Asymmetric Mannich Reaction in Organic Solvent
Open Access
- 23 October 2012
- journal article
- research article
- Published by Springer Nature in Scientific Reports
- Vol. 2 (1) , 761
Abstract
We reported the first enzyme-catalysed, direct, three-component asymmetric Mannich reaction using protease type XIV from Streptomyces griseus (SGP) in acetonitrile. Yields of up to 92% with enantioselectivities of up to 88% e.e. and diastereoselectivities of up to 92:8 (syn:anti) were achieved under the optimised conditions. This enzyme's catalytic promiscuity expands the application of this biocatalyst and provides a potential alternative method for asymmetric Mannich reactions.Keywords
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