Synergistic sodium alginate- and biochar-immobilized cells for enhancing fermentative hydrogen production from food waste

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Abstract
An immobilized hydrogen-producing consortium investigated biohydrogen production from food waste using a combination of sodium alginate and cassava rhizome biochar. We investigated the effect of varying the biochar concentration from 0 to 3% and the size of immobilized cells from 1 to 7 mm. Immobilized cells were prepared using 50% (v/v) enriched hydrogen-producing consortium, 2% (w/v) sodium alginate, and 0 to 3% (w/v) cassava rhizome biochar. The optimal conditions for achieving the highest hydrogen production in the batch fermentation reactor were identified as a biochar concentration of 2% (w/v) and an immobilized cell size of 2 mm. The highest hydrogen yield, maximum hydrogen production rate, and lag time recorded were 0.69 mmol H2/g-COD, 0.02 mmol H2/g-COD.h, and 41.51 h, respectively. This research highlights the potential of cassava biochar technology for efficient biohydrogen production from food waste, contributing to renewable energy generation and sustainable waste management.
Funding Information
  • National Science and Technology Council (NSTC 111-2221-E-035 -040 -MY3, 112-2923-E-035-001-MY3, 113-2218-E-035-002)

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