Phytochelatin Synthesis Promotes Leaf Zn Accumulation ofArabidopsis thalianaPlants Grown in Soil with Adequate Zn Supply and is Essential for Survival on Zn-Contaminated Soil
- 1 October 2016
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 57 (11) , 2342-2352
Abstract
Phytochelatin (PC) synthesis is essential for the detoxification of non-essential metals such as cadmium (Cd). In vitro experiments with Arabidopsis thaliana seedlings had indicated a contribution to zinc (Zn) tolerance as well. We addressed the physiological role of PC synthesis in Zn homeostasis of plants under more natural conditions. Growth responses, PC accumulation and leaf ionomes of wild-type and AtPCS1 mutant plants cultivated in different soils representing adequate Zn supply, Zn deficiency and Zn excess were analyzed. Growth on Zn-contaminated soil triggers PC synthesis and is strongly impaired in PC-deficient mutants. In fact, the contribution of AtPCS1 to tolerating Zn excess is comparable with that of the major Zn tolerance factor MTP1. For plants supplied with a normal level of Zn, a significant reduction in leaf Zn accumulation of AtPCS1 mutants was detected. In contrast, AtPCS1 mutants grown under Zn-limited conditions showed wild-type levels of Zn accumulation, suggesting the operation of distinct Zn translocation pathways. Contrasting phenotypes of the tested AtPCS1 mutant alleles upon growth in Zn- or Cd-contaminated soil indicated differential activation of PC synthesis by these metals. Experiments with truncated versions identified a part of the AtPCS1 protein required for the activation by Zn but not by Cd.Keywords
This publication has 49 references indexed in Scilit:
- Nicotianamine Functions in the Phloem-Based Transport of Iron to Sink Organs, in Pollen Development and Pollen Tube Growth in ArabidopsisThe Plant Cell, 2012
- Vacuolar Nicotianamine Has Critical and Distinct Roles under Iron Deficiency and for Zinc Sequestration in ArabidopsisThe Plant Cell, 2012
- Elevated Nicotianamine Levels in Arabidopsis halleri Roots Play a Key Role in Zinc HyperaccumulationThe Plant Cell, 2012
- Long-distance transport, vacuolar sequestration, tolerance, and transcriptional responses induced by cadmium and arsenicCurrent Opinion in Plant Biology, 2011
- Zinc homeostasis and signaling in health and diseasesJBIC Journal of Biological Inorganic Chemistry, 2011
- Cadmium levels in Europe: implications for human healthEnvironmental Geochemistry and Health, 2009
- Phytochelatin Synthesis Is Essential for the Detoxification of Excess Zinc and Contributes Significantly to the Accumulation of ZincPlant Physiology, 2008
- Metal movement within the plant: contribution of nicotianamine and yellow stripe 1-like transportersAnnals of Botany, 2008
- Identification of high levels of phytochelatins, glutathione and cadmium in the phloem sap of Brassica napus. A role for thiol‐peptides in the long‐distance transport of cadmium and the effect of cadmium on iron translocationThe Plant Journal, 2008
- Purdue Ionomics Information Management System. An Integrated Functional Genomics PlatformPlant Physiology, 2006