Desulfurization of dibenzothiophene by Bacillus subtilis recombinants carrying dszABC and dszD genes
Authors:
- 30 June 2006
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 28 (14) , 1095-1100
Abstract
The desulfurization (dsz) genes from Rhodococcus erythropolis DS-3 were successfully integrated into the chromosomes of Bacillus subtilis ATCC 21332 and UV1 using an integration vector pDGSDN, yielding two recombinant strains, B. subtilis M29 and M28 in which the integrated dsz genes were expressed efficiently under the promoter, Pspac. The dibenzothiophene (DBT) desulfurization efficiency of M29 was 16.2 mg DBT l−1 h−1 at 36 h, significantly higher than that of R. erythropolis DS−3 and B. subtilis M28 and also showed no product inhibition. The interfacial tension of the supernatant fermented by M29 varied from 48 mN m−1 to 4.2 mN m−1, lower than that of the recombinant strain, M28, reveals that the biosurfactant secreted from M29 may have an important function in the DBT desulfurization process.Keywords
This publication has 8 references indexed in Scilit:
- Desulfurization of 2,4,6,8-tetraethyl dibenzothiophene by recombinant Mycobacterium sp. strain MR65Biotechnology Letters, 2003
- Enhanced desulfurization in a transposon-mutant strain of Rhodococcus erythropolisBiotechnology Letters, 2003
- Isolation of a recombinant desulfurizing 4,6-Diproply dibenzothiophene in n-tetradecaneJournal of Bioscience and Bioengineering, 2003
- Recombinant Rhodococcus sp. Strain T09 Can Desulfurize DBT in the Presence of Inorganic SulfateCurrent Microbiology, 2002
- Flavin reductase coupling with two monooxygenases involved in dibenzothiophene desulfurization: purification and characterization from a non-desulfurizing bacterium, Paenibacillus polymyxa A-1Applied Microbiology and Biotechnology, 2002
- A novel enzyme, 2′-hydroxybiphenyl-2-sulfinate desulfinase (DszB), from a dibenzothiophene-desulfurizing bacterium Rhodococcus erythropolis KA2-5-1: gene overexpression and enzyme characterizationBiochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2002
- Chemostat Approach for the Directed Evolution of Biodesulfurization Gain-of-Function MutantsApplied and Environmental Microbiology, 2002
- Microbial Desulfurization of Organic Sulfur Compounds in PetroleumBioscience, Biotechnology, and Biochemistry, 1999