Capillary permeability in canine myocardium as determined by bolus injection, residue detection

Authors:
  • SHStig Haunsø
    Stig Haunsø
  • WPWilliam P. Paaske
    William P. Paaske
  • PSPer Sejrsen
    Per Sejrsen
  • OAOle Amtorp
    Ole Amtorp
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Abstract
Capillary permeability of51Cr‐EDTA in the canine myocardium was determined by applying: (A) the single injection, external registration method, and (B) the local tissue clearance method, to the intact dog heart of open‐chest anesthetized dogs. (A)51Cr‐EDTA was administered into the left anterior descending coronary artery as a bolus injection, and the response curve was recorded by external registration. The capillary diffusion capacity (the permeability‐surface area product) for51Cr‐EDTA amounted to 32.5 ml/100 g·min at a capillary extraction of 0.40 and a plasma flow of 73.3 ml/100 g·min. The diffusional permeability coefficient of51Cr‐EDTA was calculated to 1.08 × 10‐5cm/s, which indicates that the permeability of the capillaries in the myocardium for51Cr‐EDTA is similar to that of continuous capillaries in other tissues. (B)51Cr‐EDTA (3–100 μl) was injected at a depth of 5 mm into the myocardium of the left ventricular free wall and the residue curve was recorded. The capillary extraction, as determined by the tissue clearance method, was calculated to values 6 times smaller than determined with the single injection, residue detection method. This unreasonably low extraction was probably due to methodological errors inherent to the tissue clearance technique.

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