Optimization of Conditions for Bacteriocin Extraction in PEG/Salt Aqueous Two‐Phase Systems Using Statistical Experimental Designs
Authors:
- 1 January 2001
- journal article
- Published by Wiley in Biotechnology Progress
- Vol. 17 (2) , 366-368
Abstract
The bacteriocin nisin was extracted in PEG/salt aqueous two‐phase systems (ATPS) using the property that the systems can extract hydrophobic proteins. The concentrations of the phase‐forming components, PEG 4000 and Na2SO4, were optimized for nisin recovery by means of statistical experimental designs, and it was found that they strongly influenced nisin recovery. The optimal composition of ATPS was found to be 15.99% (w/w) PEG 4000 and 15.85% (w/w) Na2SO4 (pH 2), and the optimal ATPS allowed an 11.60% increase of nisin recovery compared to the standard method of nisin assay.Keywords
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