Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye
Authors:
- the Ausimmune Investigator Group (AIG)T(the Ausimmune Investigator Group (AIG)
- Meav-Lang J LayMLMeav-Lang J Lay
- Robyn M LucasRLRobyn M Lucas
- Mala RatnamohanMRMala Ratnamohan
- Janette TaylorJTJanette Taylor
- Anne-Louise PonsonbyAPAnne-Louise Ponsonby
- Dominic E DwyerDDDominic E Dwyer
Open Access
- 22 September 2010
- journal article
- research article
- Published by Springer Nature in Virology Journal
- Vol. 7 (1) , 252
Abstract
Reactivation of Epstein-Barr virus (EBV) infection may cause serious, life-threatening complications in immunocompromised individuals. EBV DNA is often detected in EBV-associated disease states, with viral load believed to be a reflection of virus activity. Two separate real-time quantitative polymerase chain reaction (QPCR) assays using SYBR Green I dye and a single quantification standard containing two EBV genes, Epstein-Barr nuclear antigen-1 (EBNA-1) and BamHI fragment H rightward open reading frame-1 (BHRF-1), were developed to detect and measure absolute EBV DNA load in patients with various EBV-associated diseases. EBV DNA loads and viral capsid antigen (VCA) IgG antibody titres were also quantified on a population sample.Keywords
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