Efficient terpene hydroxylation catalysts based upon P450 enzymes derived from Actinomycetes
- 7 July 2005
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Organic & Biomolecular Chemistry
- Vol. 3 (16) , 2930-2934
Abstract
The hydroxylation of α-ionone 1 and β-ionone 2 to their corresponding mono-hydroxylated derivatives has been examined using a recombinant E. coli whole cell system, in which cytochromes P450 SU1 and SU2, and P450 SOY were over-expressed with their cognate ferrodoxins. Both substrates are hydroxylated with a high degree of regioselectivity and for α-ionone 1 the reaction is highly diastereoselective yielding the anti-isomer.Keywords
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