Isolation and identification of bioactive compounds from Antrodia camphorata against ESKAPE pathogens

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Abstract
Antimicrobial resistance is a major threat to human health globally. Antrodia camphorata was grown in a malt/yeast extract broth liquid medium for 15 days. Then, 4-L fermentation broth was harvested, yielding 7.13 g of the ethyl acetate extract. By tracing the antimicrobial activity, 12.22 mg of the antimicrobial compound was isolated. The structure of 5-methyl-benzo [1,3]-dioxole-4,7-diol (MBBD) was elucidated using NMR and MS data analyses. The antibacterial activity of MBBD was detected through the microbroth dilution method. MBBD exhibited broad-spectrum antibacterial activity. The minimum inhibitory concentration (MIC) range of MBBD for drug-resistant pathogenic bacteria was 64–256 μg/mL, with the lowest MIC observed for Acinetobacter baumannii (64 μg/mL), followed by Pseudomonas aeruginosa (MIC = 128 μg/mL). Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli were also sensitive, with an MIC of 256 μg/mL. The MIC range of MBBD against 10 foodborne pathogens was 12.5–100 μg/mL. Based on the results of this study, MBBD exhibits broad-spectrum antibacterial activity, particularly demonstrating excellent inhibitory effects against A. baumannii. MBBD will be good candidates for new antimicrobial drugs.
Funding Information
  • General Project of the Basic Research Program in Yunnan Province (202101AT070218)
  • The National Natural Science Foundation of China (31860177)
  • The National Natural Science Foundation of China (32160736)
  • General Project of the Basic Research Program in Yunnan Province (202101AT070044)
  • Major Project of the Agricultural Basic Research Program in Yunnan Province (202101BD070001-020)
  • the Reserve Talents for Young and Middle-aged Aca-demic and Technical Leaders of the Yunnan Province (202205AC160044)
  • the Yunnan Talent Support Program Young Talent (XDYC-QNRC-2022-0245)
  • Yunnan Key Laboratory for Fungal Diversity and Green Development (E03A311261-3)

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