Cis- and cell-type-dependent trans-requirements for Lassa virus-like particle production
Authors:
- 1 July 2015
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 96 (7) , 1626-1635
Abstract
Lassa virus (LASV) small zinc-finger protein (Z), which contains two L-domain motifs, plays a central role in virus budding. Here, we report that co-expression of glycoprotein (GPC) altered the requirements for cholesterol but not the L-domains and host factor, Tsg101, for Z-induced virus-like particle (VLP) production. In particular, the cholesterol requirement for VLP production was cell-type-dependent. In addition, GPC was found to be important for co-localization of Z with CD63, a late endosomal marker. We also found that the N-terminal region (aa 3-10) of Z was critical for its myristoylation and VLP production. These findings will contribute to our understanding of LASV assembly and budding.Keywords
This publication has 40 references indexed in Scilit:
- Arenavirus reverse genetics: New approaches for the investigation of arenavirus biology and development of antiviral strategiesVirology, 2011
- Assembly of Arenavirus Envelope Glycoprotein GPC in Detergent-Soluble Membrane MicrodomainsJournal of Virology, 2009
- The RING Domain and the L79 Residue of Z Protein Are Involved in both the Rescue of Nucleocapsids and the Incorporation of Glycoproteins into Infectious Chimeric Arenavirus-Like ParticlesJournal of Virology, 2009
- Mechanisms for enveloped virus budding: Can some viruses do without an ESCRT?Virology, 2008
- Arenavirus Z-Glycoprotein Association Requires Z Myristoylation but Not Functional RING or Late DomainsJournal of Virology, 2007
- Late budding domains and host proteins in enveloped virus releaseVirology, 2006
- Cells Expressing the RING Finger Z Protein Are Resistant to Arenavirus InfectionJournal of Virology, 2004
- Characterization of the Arenavirus RING Finger Z Protein Regions Required for Z-Mediated Inhibition of Viral RNA SynthesisJournal of Virology, 2002
- RING Finger Z Protein of Lymphocytic Choriomeningitis Virus (LCMV) Inhibits Transcription and RNA Replication of an LCMV S-Segment MinigenomeJournal of Virology, 2001
- Myristoylation-dependent replication and assembly of human immunodeficiency virus 1.Proceedings of the National Academy of Sciences, 1990