Combined fluorescence and phosphorescence lifetime imaging
- 29 February 2016
- journal article
- conference paper
- Published by AIP Publishing in Applied Physics Letters
- Vol. 108 (9) , 091111
Abstract
We present a lifetime imaging technique that simultaneously records the fluorescence and phosphorescence lifetime images in confocal laser scanning systems. It is based on modulating a high-frequency pulsed laser synchronously with the pixel clock of the scanner, and recording the fluorescence and phosphorescence signals by multidimensional time-correlated single photon counting board. We demonstrate our technique on the recording of the fluorescence/phosphorescence lifetime images of human embryonic kidney cells at different environmental conditions.Keywords
Funding Information
- Russian Foundation for Basic Research (15-32-20324)
This publication has 25 references indexed in Scilit:
- Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurementScientific Reports, 2015
- Two-photon high-resolution measurement of partial pressure of oxygen in cerebral vasculature and tissueNature Methods, 2010
- Dendritic Phosphorescent Probes for Oxygen Imaging in Biological SystemsACS Applied Materials & Interfaces, 2009
- Lanthanide Complexes and Quantum Dots: A Bright Wedding for Resonance Energy TransferEuropean Journal of Inorganic Chemistry, 2008
- Principles of Fluorescence Spectroscopy2006
- Evaluation of Phosphorescent Rhenium and Iridium Complexes in Polythionylphosphazene Films for Oxygen Sensor ApplicationsChemistry of Materials, 2005
- Divalent Osmium Complexes: Synthesis, Characterization, Strong Red Phosphorescence, and ElectrophosphorescenceJournal of the American Chemical Society, 2002
- Microvascular and interstitial Po2measurements in rat skeletal muscle by phosphorescence quenchingJournal of Applied Physiology, 2001
- Fluorescence lifetime imaging of oxygen in living cellsJournal of Fluorescence, 1997
- A Versatile and Sensitive Method for Measuring Oxygen1987