High-Yield Activation of Scaffold Polymer Surfaces To Attach Cell Adhesion Molecules
- 14 December 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 129 (1) , 93-97
Abstract
Zirconium tetra(tert-butoxide) reacts with surface amide groups of polyamide nylon 6/6 to give (η2-amidate)zirconium complexes in high yield. These surface complexes react to bond the cell-adhesive peptide arginine-glycine-aspartic acid (RGD) to the polymer surface. A surface loading of 0.18 nmol/cm2 of RGD is achieved, which is 20−1000 times higher than previously reported attainable on natural or synthetic polymers by other strategies. Approximately 40% of the nylon surface is covered by the RGD, which gives a surface that is both stable to hydrolysis and highly active for cell adhesion and spreading in vitro.Keywords
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