Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Authors:
Publisher website
Google Scholar
Abstract
Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β‐1,3‐glucanase from banana (Musa acuminata), denoted as Mus a 5, were performedMethods and results The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI‐TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N‐terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration‐dependent mannerConclusion Mus a 5 is a functional allergen and a candidate for the component‐resolved allergy diagnosis of banana allergy.
Funding Information
  • Ministry of Education and Science, Republic of Serbia (172049)

This publication has 36 references indexed in Scilit: