High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9
- 12 September 2023
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 42 (12) , 2039-2042
Abstract
Key message 4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.Keywords
Funding Information
- Creation and Identification of Editing Material Library of Tobacco Transporter and Enzyme Related Genes in Red flower Mammoth Gold (110202101034)
- Development of Tagless Gene Editing Technology and Breeding Application of Low Nicotine Editing Materials (2022JY03)
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