CRISPR-Cas
Authors:
Open Access
- 7 February 2013
- journal article
- review article
- Published by Taylor & Francis in RNA Biology
- Vol. 10 (5) , 679-686
Abstract
The CRISPR-Cas (clustered regularly interspaced short palindromic repeats, CRISPR-associated genes) is an adaptive immunity system in bacteria and archaea that functions via a distinct self-non-self recognition mechanism that is partially analogous to the mechanism of eukaryotic RNA interference (RNAi). The CRISPR-Cas system incorporates fragments of virus or plasmid DNA into the CRISPR repeat cassettes and employs the processed transcripts of these spacers as guide RNAs to cleave the cognate foreign DNA or RNA. The Cas proteins, however, are not homologous to the proteins involved in RNAi and comprise numerous, highly diverged families. The majority of the Cas proteins contain diverse variants of the RNA recognition motif (RRM), a widespread RNA-binding domain. Despite the fast evolution that is typical of the cas genes, the presence of diverse versions of the RRM in most Cas proteins provides for a simple scenario for the evolution of the three distinct types of CRISPR-cas systems. In addition to severa...Keywords
This publication has 70 references indexed in Scilit:
- Comprehensive analysis of the HEPN superfamily: identification of novel roles in intra-genomic conflicts, defense, pathogenesis and RNA processingBiology Direct, 2013
- Strong bias in the bacterial CRISPR elements that confer immunity to phageNature Communications, 2013
- Mechanism of Foreign DNA Selection in a Bacterial Adaptive Immune SystemMolecular Cell, 2012
- CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3Molecular Cell, 2012
- Structure and Mechanism of the CMR Complex for CRISPR-Mediated Antiviral ImmunityMolecular Cell, 2012
- CRISPR RNA maturation by trans-encoded small RNA and host factor RNase IIINature, 2011
- Cas3 is a single‐stranded DNA nuclease and ATP‐dependent helicase in the CRISPR/Cas immune systemThe EMBO Journal, 2011
- Interaction of the Cas6 Riboendonuclease with CRISPR RNAs: Recognition and CleavageStructure, 2011
- RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein ComplexCell, 2009
- Structural Basis for DNase Activity of a Conserved Protein Implicated in CRISPR-Mediated Genome DefenseStructure, 2009