Decay‐accelerating factor CD55 is identified as the receptor for echovirus 7 using CELICS, a rapid immuno‐focal cloning method.
Authors:
Open Access
- 1 November 1994
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 13 (21) , 5070-5074
Abstract
Using an anti‐receptor mAb that blocks the attachment of echovirus 7 and related viruses (echoviruses 13, 21, 29 and 33), we have isolated a complementary DNA clone that encodes the human decay‐accelerating factor (CD55). Mouse cells transfected with the CD55 clone bind echovirus 7, and this binding is blocked by the anti‐receptor mAb. The method used (CELICS) allows rapid and direct cloning of genes encoding cell surface receptors. It is based on episomal replication and high efficiency expression of complementary DNA clones in the vector pCDM8 in COS or WOP cells, in conjunction with a sensitive immuno‐focal screen that uses antibody probes linked to beta‐galactosidase. Receptor positive cells were identified by a colour change and isolated individually using a micromanipulator. DNA extracted from a small number of cells was then cloned directly in Escherichia coli.Keywords
This publication has 33 references indexed in Scilit:
- Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on resting leukocytesNature, 1992
- Expression cloning of a diphtheria toxin receptor: Identity with a heparin-binding EGF-like growth factor precursorCell, 1992
- Expression cloning of the murine erythropoietin receptorCell, 1989
- The major human rhinovirus receptor is ICAM-1Cell, 1989
- cDNA Expression Cloning of the IL-1 Receptor, a Member of the Immunoglobulin SuperfamilyScience, 1988
- The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brainCell, 1986
- Human GM-CSF: Molecular Cloning of the Complementary DNA and Purification of the Natural and Recombinant ProteinsScience, 1985
- Monoclonal antibodies which block cellular receptors of poliovirusVirus Research, 1984
- The Preparation of Specific Immune Sera against Type 3 Poliovirus D-Antigen and C-Antigen and the Demonstration of Two C-Antigenic Components in Vaccine Strain PopulationsJournal of General Virology, 1980
- Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology, 1967