Protein hydrolysates in cell culture: Toward multi‐omics characterization

Authors:
Open Access
Publisher website
Google Scholar
Abstract
The high cost of conventional cell culture media has driven demand for sustainable, cost‐effective serum‐free formulations in biopharmaceutical manufacturing and cultivated meat production. Although plant‐ and yeast‐derived hydrolysates are generally considered to be promising additives, significant variability in raw materials and processing conditions results in substantial compositional and batch‐to‐batch differences between products, making it challenging to predict effectiveness in cell culture a priori . This review examines hydrolysate media additives through the specific lens of comprehensive multi‐omic characterization as a means of addressing this uncertainty. While typical characterization is often limited to metrics such as degree of hydrolysis or color, detailed metabolomic and peptidomic profiling remains uncommon. We identify a gap in systematic, high‐resolution characterization and argue that integrating compositional and bioactivity analyses is essential to transition hydrolysates from undefined inputs toward semi‐defined media components.
Funding Information
  • Dalhousie University
  • Good Food Institute (21‐CM‐CA‐EG‐1‐04)
  • Natural Sciences and Engineering Research Council of Canada (RGPIN‐2019‐04694)
  • Mitacs (IT43001)

This publication has 97 references indexed in Scilit: