GAS-LIQUID CHROMATOGRAPHIC-MASS SPECTROMETRIC DETERMINATION OF ALPHA-NAPHTHYLAMINE AND BETA-NAPHTHYLAMINE IN FD AND C RED NO 2 (AMARANTH)

Authors:
  • BSB Stavric
    B Stavric
  • RKR Klassen
    R Klassen
  • WMW Miles
    W Miles
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  • 1 January 1979
    • journal article
    • research article
    • Vol. 62  (5) , 1020-1026
    • PMID:528442
Abstract
A method is described for the simultaneous quantitation of trace amounts of .alpha.- (.alpha.-NA) and .beta.-naphthylamines (.beta.-NA) with detectability in 0.1 ppb range and sensitivity of 50 pmol in certified food grade amaranth (FD and C [food drug and cosmetics] Red No. 2; C.I. Food Red 9; CI 16185). The amaranth sample is extracted with benzene and the evaporated residue is derivatized with perfluorooctanoic anhydride. The resulting derivatives are separated by GLC and identified and quantitated by mass spectrometric monitoring of the m/e at 539.04. The method was used for quantitation of .alpha.-NA and .beta.-NA in randomly chosen samples of amaranth. Of 11 samples from different manufacturers, 5 were free of the .beta.-isomer; the remaining samples contained up to 1.2 ppb .beta.-NA. The concentration of .alpha.-NA ranged from no detectable amount to 970 ppb; the majority of the samples contained less than 7 ppb.