METFORMIN AND DICHOLOROACETATE COMBINATION EXERT A SYNERGISTIC EFFECT ON CELL VIABILITY OF ORAL SQUAMOUS CELL CARCINOMA
Authors:
- Seniz InancSISeniz Inanc
- Didem KelesDKDidem Keles
- Gorkem EskiizmirGEGorkem Eskiizmir
- Yasemin BasbinarYBYasemin Basbinar
- Gulgun OktayGOGulgun Oktay
Open Access
- 29 May 2019
- journal article
- research article
- Published by UK Scientific Publishing Limited in ENT Updates
- Vol. 9 (2) , 68-73
Abstract
Objective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line. Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x10 4 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability. In addition, drug combination index was analyzed with CompuSyn software according to Chou-Talalay method. Results: Half-maximal inhibitory concentrations (IC 50 ) of Metformin and DCA were found to be 3mM and 23mM, respectively, for 72 hours. CI values (0.76-0.80) in all combination groups below 1 indicated that Metformin/DCA combination had a moderate synergistic effect on cell viability in UPCI-SCC-131 cells. Discussion: Metformin/DCA combination synergistically decreased the cell viability of UPCI-SCC-131 cells. Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the " drug repositioning " of the treatment of oral cavity cancer.Keywords
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