Plug-and-Play Metabolic Transducers Expand the Chemical Detection Space of Cell-Free Biosensors
Preprint
Abstract
Cell-free transcription-translation systems have great potential for biosensing, yet the range of detectable chemicals is limited. Here we provide a framework to expand the range of molecules detectable by cell-free biosensors by combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors are highly-sensitive and have a fast response and high-dynamic range. This work provides a foundation to engineer modular cell-free biosensors tailored for many applications.Keywords
All Related Versions
- Published version: Nature Communications, 10 (1), 1.
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