Determination of cephalosporins utilising thin-layer chromatography with fluorescamine detection

Authors:
  • HFHuguette Fabre
    Huguette Fabre
  • MBMarie-Dominique Blanchin
    Marie-Dominique Blanchin
  • DLDan Lerner
    Dan Lerner
  • BMBernadette Mandrou
    Bernadette Mandrou
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Abstract
A thin-layer chromatographic procedure utilising fluorescamine detection is described for the quantitation of cephradine, cefaclor, cephalexin and cefadroxil. The repeatability, sensitivity and detectability of the method are compared with those for o-phthalaldehyde fluorescence emission and UV absorption measurements on the plate. The applicability of the fluorescamine method to the determination of these cephalosporins in serum, urine and pharmaceutical formulations is discussed.