Indirect Competitive Aptamer-Based Enzyme-Linked Immunosorbent Assay (apt-ELISA) for the Specific and Sensitive Detection of Isocarbophos Residues

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Abstract
Environmental contamination from pesticide residues is a worldwide public health concern and has attracted increasing attention from researchers over the past several years. In this study, an aptamer-based enzyme-linked immunosorbent assay (apt-ELISA) using enzyme signal amplification has been developed to determine isocarbophos residues. The limit of detection for the apt-ELISA was 0.05 ng/mL for isocarbophos, and the sensitivity was better than that obtained using the traditional ELISA approach. The average recovery of the apt-ELISA ranged from 84 to 97%, and the coefficient of variation ranged from 4.6 to 12.1% when determining the isocarbophos residues in various water samples. The results demonstrate that the proposed approach can be used for routine screening of isocarbophos residues.
Funding Information
  • the Project Financed by the International Science and Technology Cooperation Plan of Anhui Province (225)
  • the National Science Foundation of China (21205001, 41471422)
  • the High-Efficiency Humanities and Social Sciences research projects of Anhui Province (ZD043)

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