Two Dimensional Patterning of Fluorescent Proteins in Hydrogels
- 8 December 2005
- journal article
- letter
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 22 (1) , 29-31
Abstract
This work describes the successful micropatterning of hybrid systems consisting of hydrogel-dispersed optically active and controllable proteins on solid surfaces without degradation of the photophysical properties of the light-emitting biomolecules. It demonstrates the preservation of the luminescence properties of proteins entrapped into isolated microstructures of poly(acrylamide) gel. This way we can exploit both the structural and function-preserving properties of the hydrogels and the functionality of light-emitting proteins. We believe that this approach can open the way to the realization of nanopatterned optical memories based on photochromic biomolecules.Keywords
This publication has 12 references indexed in Scilit:
- Conformation of Microcontact-Printed Proteins by Atomic Force Microscopy Molecular SizingLangmuir, 2005
- Mechanical perturbation-induced fluorescence change of green fluorescent proteinApplied Physics Letters, 2005
- Multiphoton switching dynamics of single green fluorescent proteinsPhysical Review E, 2004
- Photoreversible Dark State in a Tristable Green Fluorescent Protein VariantThe Journal of Physical Chemistry B, 2003
- Green Fluorescent Protein (GFP): Applications, Structure, and Related Photophysical BehaviorChemical Reviews, 2002
- Visualization of in Vivo Direct Interaction between HIV-1 TAT and Human Cyclin T1 in Specific Subcellular Compartments by Fluorescence Resonance Energy TransferJournal of Biological Chemistry, 2001
- Frequency Tuning of Basilar Membrane and Auditory Nerve Fibers in the Same Cochleae1998
- Green Fluorescent Protein as a Noninvasive Intracellular pH IndicatorBiophysical Journal, 1998
- Improved green fluorescenceNature, 1995
- Green Fluorescent Protein as a Marker for Gene ExpressionScience, 1994