Ultrastructure of Resting Cells of Some Non-Spore-Forming Bacteria

Authors:
  • NSN. E. Suzina
    N. E. Suzina
  • AMA. L. Mulyukin
    A. L. Mulyukin
  • AKA. N. Kozlova
    A. N. Kozlova
  • ASA. P. Shorokhova
    A. P. Shorokhova
  • VDV. V. Dmitriev
    V. V. Dmitriev
  • EBE. S. Barinova
    E. S. Barinova
  • OMO. N. Mokhova
    O. N. Mokhova
  • GEG. I. El'-Registan
    G. I. El'-Registan
  • VDV. I. Duda
    V. I. Duda
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Abstract
Using electron microscopy (ultrathin sections and freeze-fractures), we investigated the ultrastructure of the resting cells formed in cultures of Micrococcus luteus, Arthrobacter globiformis, and Pseudomonas aurantiaca under conditions of prolonged incubation (up to 9 months). These resting cells included cystlike forms that were characterized by a complex cell structure and the following ultrastructural properties: (i) a thickened or multiprofiled cell wall (CW), typically made up of a layer of the preexisting CW and one to three de novo synthesized murein layers; (ii) a thick, structurally differentiated capsule; (iii) the presence of large intramembrane particles (d = 180–270 Å), occurring both on the PF and EF faces of the membrane fractures of M. luteus and A. globiformis; (iv) a peculiar structure of the cytoplasm, which was either fine-grained or lumpy (coarse-grained) in different parts of the cell population; and (v) a condensed nucleoid. Intense formation of cystlike cells occurred in aged (2- to 9-month-old) bacterial cultures grown on diluted complex media or on nitrogen-, carbon-, and phosphorus-limited synthetic media, as well as in cell suspensions incubated in media with sodium silicate. The general morphological properties, ultrastructural organization, and physiological features of cystlike cells formed during the developmental cycle suggest that constitutive dormancy is characteristic of non-spore-forming bacteria.

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