Practical Preparation of Epilactose Produced with Cellobiose 2-Epimerase fromRuminococcus albusNE1
Authors:
- 23 August 2010
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 74 (8) , 1736-1737
Abstract
A practical purification method for a non-digestible disaccharide, epilactose (4-O-β-galactosyl-D-mannose), was established. Epilactose was synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of lactose by crystallization, (ii) hydrolysis of lactose by β-galactosidase, (iii) digestion of monosaccharides by yeast, and (iv) column chromatography with Na-form cation exchange resin. Epilactose of 91.1% purity was recovered at 42.5% yield.Keywords
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