Harnessing Dye-Induced Local Heating in Lipid Membranes: A Path to Near-Infrared Light-Modulated Artificial Synaptic Vesicles

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Abstract
Optical heating coupled with near-infrared (NIR) light and photothermal materials enables local heating within biospecimens, minimizing undesirable thermal damage. Here, we demonstrated that photothermally heating lipid bilayers embedded with a phthalocyanine dye (VPc) efficiently perturbs the bilayers, resulting in increased permeability. Notably, microscopic studies revealed that the changes in membrane permeability may not follow the conventional mechanism of temperature-sensitive liposomes, which involve a bulk temperature increase that induces a phase transition across the entire lipid bilayer. Furthermore, the heat generated by NIR laser illumination rarely diffused into the surrounding environment, and the dye was located within the bilayers at the molecular level, where it effectively transferred heat to the lipid bilayer. We prepared VPc-embedded liposomes encapsulating acetylcholine (ACh) and demonstrated the NIR laser-triggered release of ACh, creating a concentration jump across a few cells or within a limited single cell region. This method induced Ca2+ flux through ACh receptor stimulation in thermally delicate biospecimens such as C2C12 myotubes and the Drosophila brain.
Funding Information
  • Asahi Glass Foundation
  • Japan Society for the Promotion of Science (20K22525, 21K19044)
  • Fusion Oriented REsearch for disruptive Science and Technology (JPMJFR201E)
  • Support for Pioneering Research Initiated by the Next Generation (JPMJSP2135)
  • World Premier International Research Center Initiative Nano Life Science Institute

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