Functional characterization of human pluripotent stem cell-derived arterial endothelial cells

Authors:
Publisher website
Google Scholar
Abstract
Here, we report the derivation of arterial endothelial cells from human pluripotent stem cells that exhibit arterial-specific functions in vitro and in vivo. We combine single-cell RNA sequencing of embryonic mouse endothelial cells with an EFNB2-tdTomato/EPHB4-EGFP dual reporter human embryonic stem cell line to identify factors that regulate arterial endothelial cell specification. The resulting xeno-free protocol produces cells with gene expression profiles, oxygen consumption rates, nitric oxide production levels, shear stress responses, and TNFα-induced leukocyte adhesion rates characteristic of arterial endothelial cells. Arterial endothelial cells were robustly generated from multiple human embryonic and induced pluripotent stem cell lines and have potential applications for both disease modeling and regenerative medicine.
Funding Information
  • The Charlotte Geyer Foundation (––)
  • HHS | National Institutes of Health (NIH 1UH2TR000506-01)
  • HHS | National Institutes of Health (NIH R21EB016381-01)
  • HHS | National Institutes of Health (3UH2TR000506-02S1)
  • HHS | NIH | National Heart, Lung, and Blood Institute (U01HL134655)

This publication has 46 references indexed in Scilit: