The complete genome sequence and genetic analysis of ΦCA82 a novel uncultured microphage from the turkey gastrointestinal system
Open Access
- 29 June 2011
- journal article
- research article
- Published by Springer Nature in Virology Journal
- Vol. 8 (1) , 331
Abstract
The genomic DNA sequence of a novel enteric uncultured microphage, ΦCA82 from a turkey gastrointestinal system was determined utilizing metagenomics techniques. The entire circular, single-stranded nucleotide sequence of the genome was 5,514 nucleotides. The ΦCA82 genome is quite different from other microviruses as indicated by comparisons of nucleotide similarity, predicted protein similarity, and functional classifications. Only three genes showed significant similarity to microviral proteins as determined by local alignments using BLAST analysis. ORF1 encoded a predicted phage F capsid protein that was phylogenetically most similar to the Microviridae ΦMH2K member's major coat protein. The ΦCA82 genome also encoded a predicted minor capsid protein (ORF2) and putative replication initiation protein (ORF3) most similar to the microviral bacteriophage SpV4. The distant evolutionary relationship of ΦCA82 suggests that the divergence of this novel turkey microvirus from other microviruses may reflect unique evolutionary pressures encountered within the turkey gastrointestinal system.Keywords
This publication has 42 references indexed in Scilit:
- Identification, sequencing and molecular analysis of Chp4, a novel chlamydiaphage of Chlamydophila abortus belonging to the family MicroviridaeJournal of General Virology, 2011
- The prospect for bacteriophage therapy in Western medicineNature Reviews Drug Discovery, 2003
- Marine phage genomicsComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2002
- Common themes among bacteriophage-encoded virulence factors and diversity among the bacteriophages involvedTrends in Microbiology, 2002
- Genomic analysis of uncultured marine viral communitiesProceedings of the National Academy of Sciences, 2002
- Bacteriophage Control of Bacterial VirulenceInfection and Immunity, 2002
- Microviridae , a Family Divided: Isolation, Characterization, and Genome Sequence of φMH2K, a Bacteriophage of the Obligate Intracellular Parasitic Bacterium Bdellovibrio bacteriovorusJournal of Bacteriology, 2002
- Comparative phage genomics and the evolution of Siphoviridae: insights from dairy phagesMolecular Microbiology, 2001
- Microvirus of Chlamydia psittaci strain Guinea pig Inclusion Conjunctivitis: isolation and molecular characterization The GenBank accession number for the sequence reported in this paper is U41758.Microbiology, 2000
- Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39Nucleic Acids Research, 2000