Chemical and biological properties of an extracellular lipopolysaccharide from Escherichia coli grown under lysine-limiting conditions

Authors:
  • ATA Taylor
    A Taylor
  • KKKw Knox
    Kw Knox
  • EWE Work
    E Work
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Abstract
Lipopolysaccharide was prepared from the extracellular lipoglyco-peptide produced by the lysine-requiring mutant E. coli A.T.C.C. 12408 grown under lysine-limiting conditions. The lipid moiety, containing glucosamine phosphate and four fatty-acids (lauric acid, myristic acid, [beta]-hydroxymyristic acid and palmitic acid) corresponded in composition to lipid-A of known bacterial lipopolysaccharides. The components of the polysaccharide moiety were D-glucose, D-galactose, L-glycero-D-manno-heptose, 3-deoxy-2-oxo-octonic acid, ethanolamine and phosphate. These are the constituents of the polysaccharide of the cell-wall antigens from rough strains of E. coli. Lipopolysaccharides were also prepared from whole cells of E. coli 12408 grown with excess or limited amounts of lysine; they were identical in carbohydrate composition with the extracellular lipopolysaccharide. The biological properties of this material also resembled those of known lipopolysaccharides; it was antigenic, pyrogenic, toxic and had adjuvant activity.

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