L-Tyrosine-3, 5- 3 H Assay for Tyrosinase Development in Skin of Newborn Hamsters

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Abstract
Tyrosinase in hamster skin, measured by the production of 3 HOH from L-tyrosine-3,5- 3 H, increases about sevenfold in specific activity from the 14-day fetus to day 6 of life. Albino hamsters exhibit no activity, but enzyme is present in the ventral (white) areas of the Syrian hamster. 3,4-Dihydroxy-L-phenylalanine is required as a cosubstrate.

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