The Thiol Proteinases from the Latex ofCarica papayaL. I. Fractionation, Purification and Preliminary Characterization
Authors:
- Alain JacquetAJAlain Jacquet
- Arthur Georges SchnekASArthur Georges Schnek
- Yvan LoozeYLYvan Looze
- 1 January 1988
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 369 (2) , 733-740
Abstract
Three thiol proteinases, namely papain, chymopapain and proteinase .OMEGA. were purified to homogeneity from the latex of Carica papaya L. During the purification procedure, the thiol function of the cysteinyl residues were protected either as mixed disulfides with cysteamine or 2-thiopyridone or as S-sulphenylthiosulfate derivative or after blocking with p-chloromercuribenzoic acid. In marked contrast with earlier publications, chymopapain also was found to be a monothiol proteinase as papain and proteinase .OMEGA.. The active site of chymopapain and proteinase .OMEGA. could not be distinguished from that of papain neither by the analysis of the pH dependence of Kcat/Km nor by the examination of the pH dependence of the fluorescence emission spectra.Keywords
This publication has 8 references indexed in Scilit:
- The Thiol Proteinases from the Latex ofCarica papayaL. II. The Primary Structure of Proteinase ΩBiological Chemistry Hoppe-Seyler, 1988
- High-performance liquid chromatographic investigations on some enzymes of papaya latexJournal of Chromatography A, 1985
- Evidence from two-protonic-state reactivity probe kinetics that chymopapain in fresh nonfruit latex ofCarica papaya consists of multiple forms of chymopapain A. The value of catalytic site characteristics in the identification, classification, and characterization of the papaya cysteine proteinases papain, the chymopapains, and papaya proteinase ΩThe Protein Journal, 1985
- Structure of papain refined at 1.65 Å resolutionJournal of Molecular Biology, 1984
- Purification and characterization of a novel proteinase, chymopapain SBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- The Complete Amino Acid Sequence of PapainJournal of Biological Chemistry, 1970
- Spectroscopic Studies on Papain and Some Inactive DerivativesJournal of Biological Chemistry, 1969
- The preparation and properties of two new chromogenic substrates of trypsinArchives of Biochemistry and Biophysics, 1961