The Polypeptides of Hepatitis A Virus

Authors:
  • ACAnthony G. Coulepis
    Anthony G. Coulepis
  • SLStephen A. Locarnini
    Stephen A. Locarnini
  • AFAllan A. Ferris
    Allan A. Ferris
  • NLNoreen I. Lehmann
    Noreen I. Lehmann
  • IGIan D. Gust
    Ian D. Gust
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Abstract
Hepatitis A virus was purified from fecal specimens obtained from 3 patients with naturally acquired hepatitis A, by a process of differential centrifugation, chloroform extraction, column chromatography, and isopycnic ultracentrifugation. Analysis of purified virus by discontinuous SDS-PAGE revealed three major polypeptides with molecular weights of 34,000, 25,500, and 23,000 daltons. These polypeptides appear to be specific for hepatitis A virus and have similar molecular weights to three of the four major polypeptides reported for members of the genus Enterovirus within the family Picornaviridae.