Construction of pET-32 α (+) vector for protein expression and purification
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Open Access
- 1 January 2012
- journal article
- research article
- Published by Medknow in North American Journal of Medical Sciences
- Vol. 4 (12) , 651-5
Abstract
The construction of expression vector is a basic tool for biotechnology and production of desired proteins, this article summarized the construction of pET-32 α (+) vector techniques which are generally used in research laboratories. The procedures include that acquisition of the exogenous DNA fragment for construction of the vector, subcloning the DNA fragment into pET-32 α (+) expression vector, protein expression in Escherichia coli BL21 (DE3) and protein purification under native conditions in E. coli lysates.Keywords
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