The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop fold
Open Access
- 4 March 2011
- journal article
- Published by Wiley in FEBS Letters
- Vol. 585 (7) , 1049-1053
Abstract
The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is regulated by specific viral genome sequences within 5'- and 3'-untranslated regions (5'-UTR and 3'-UTR). Here we report the solution structure of 5'-UTR derived stem-loop 2 (SL2) of SARS-CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5-bp stem and adopts a canonical CUYG tetraloop fold with the 3' nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed.Keywords
This publication has 30 references indexed in Scilit:
- The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop foldFEBS Letters, 2011
- Thermodynamic Characterization of RNA TriloopsBiochemistry, 2010
- Mouse Hepatitis Virus Stem-Loop 2 Adopts a uYNMG(U)a-Like Tetraloop Structure That Is Highly Functionally Tolerant of Base SubstitutionsJournal of Virology, 2009
- 3DNA: a versatile, integrated software system for the analysis, rebuilding and visualization of three-dimensional nucleic-acid structuresNature Protocols, 2008
- Structural Lability in Stem–Loop 1 Drives a 5′ UTR–3′ UTR Interaction in Coronavirus ReplicationJournal of Molecular Biology, 2008
- Genetic Interactions between an Essential 3′ cis -Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3′ End of the Mouse Coronavirus GenomeJournal of Virology, 2008
- A Hypervariable Region within the 3′ cis -Acting Element of the Murine Coronavirus Genome Is Nonessential for RNA Synthesis but Affects PathogenesisJournal of Virology, 2007
- Putative cis -Acting Stem-Loops in the 5′ Untranslated Region of the Severe Acute Respiratory Syndrome Coronavirus Can Substitute for Their Mouse Hepatitis Virus CounterpartsJournal of Virology, 2006
- Rewiring the severe acute respiratory syndrome coronavirus (SARS-CoV) transcription circuit: Engineering a recombination-resistant genomeProceedings of the National Academy of Sciences, 2006
- NMRPipe: A multidimensional spectral processing system based on UNIX pipesJournal of Biomolecular NMR, 1995