Monitoring H2O2on the Surface of Single Cells with Liquid Crystal Elastomer Microspheres

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Abstract
Live‐imaging of signaling molecules released from living cells is a fundamental challenge in life sciences. Herein, we synthesized liquid crystal elastomer microspheres functionalized with horse‐radish peroxidase (LCEM‐HRP), which can be immobilized directly on the cell membrane to monitor real‐time release of H2O2at the single‐cell level. LCEM‐HRP could report H2O2through a concentric‐to‐radial (C‐R) transfiguration, which is due to the deprotonation of LCEM‐HRP and the break of inter or intra‐chain hydrogen bonding in LCEM‐HRP caused by HRP‐catalyzed reduction of H2O2. The level of transfiguration of LCEM‐HRP revealed the different amounts of H2O2released from cells. The estimated detection sensitivity was ≈2.2×10−7 μmfor 10 min of detection time. The cell lines and cell–cell heterogeneity was explored from different configurations. LCEM‐HRP presents a new approach for in situ real‐time imaging of H2O2release from living cells and can be the basis for seeking more advanced chemical probes for imaging of various signaling molecules in the cellular microenvironment.
Funding Information
  • National Natural Science Foundation of China (21804026, 21727814, 21621003)

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