Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
Authors:
- Sharlaa Badal-FaesenSBSharlaa Badal-Faesen
- Cynthia FirnhaberCFCynthia Firnhaber
- Michelle A. KendallMKMichelle A. Kendall
- Xingye WuXWXingye Wu
- Beatriz GrinsztejnBGBeatriz Grinsztejn
- Rodrigo Otavio da Silva EscadaRERodrigo Otavio da Silva Escada
- Michel FernandezMFMichel Fernandez
- Evelyn HoggEHEvelyn Hogg
- Ian SanneISIan Sanne
- Pamela JohnsonPJPamela Johnson
- David AllandDADavid Alland
- Gerald H. MazurekGMGerald H. Mazurek
Open Access
- 7 August 2017
- journal article
- research article
- Published by MDPI AG in Journal of Clinical Medicine
- Vol. 6 (8) , 78
Abstract
As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8).Keywords
This publication has 7 references indexed in Scilit:
- Evaluation of Xpert MTB/RIF Versus AFB Smear and Culture to Identify Pulmonary Tuberculosis in Patients With Suspected Tuberculosis From Low and Higher Prevalence SettingsClinical Infectious Diseases, 2016
- Xpert® MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adultsCochrane Database of Systematic Reviews, 2014
- Comparison of Overnight Pooled and Standard Sputum Collection Method for Patients with Suspected Pulmonary Tuberculosis in Northern TanzaniaTuberculosis Research and Treatment, 2012
- Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation studyThe Lancet, 2011
- Rapid Molecular Detection of Tuberculosis and Rifampin ResistanceNew England Journal of Medicine, 2010
- Better patient instruction for sputum sampling can improve microscopic tuberculosis diagnosis.2005
- A Minimum 5.0 ml of Sputum Improves the Sensitivity of Acid-fast Smear for Mycobacterium tuberculosisAmerican Journal of Respiratory and Critical Care Medicine, 2000