Effects of lipoxin A4 on chemotaxis and degranulation of human eosinophils stimulated by platelet‐activating factor and N‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine

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Abstract
Lipoxins are trihydroxytetraene metabolites derived through a double lipoxygenation of arachidonic acid. Lipoxin A4 (LXA4) was prepared by total chemical synthesis, and its capacity to modulate eosinophil migration has been evaluated. LXA4 is a weak and partial chemotactic agent; at 10−6 M, it achieved about 20% of the response of 10−6 M platelet‐activating factor (PAF). Preincubation of eosinophils with increasing doses of LXA4 (10−10−10−5 M) resulted in a concentration‐dependent inhibition of cell migration induced by 10‐6 M formyl‐methionyl‐leucyl‐phenylalanine (FMLP) and 10‐6 M PAF. The concentration of LXA4 which produced 50% inhibition (IC50) of eosinophil migration was approximately 10‐6 M. LXA4 (10‐10‐10‐6 M) did not elicit ECP release or modulate ECP release induced by 10‐6 M FMLP. LXA4 may have antiallergic properties in preventing eosinophilic migration.

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