Exercise training decreases the size and alters the composition of the neointima in a porcine model of percutaneous transluminal coronary angioplasty (PTCA)
Authors:
- Douglas K. BowlesDBDouglas K. Bowles
- M. Harold LaughlinMLM. Harold Laughlin
- Dirk J. DunckerDDDirk J. Duncker
- Michael SturekMSMichael Sturek
- Xin LongXLXin Long
- Ian N. BratzIBIan N. Bratz
- Mouhamad AllooshMAMouhamad Alloosh
- Jason M. EdwardsJEJason M. Edwards
Open Access
- 1 September 2009
- journal article
- research article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 107 (3) , 937-945
Abstract
Exercise training (EX) following percutaneous transluminal coronary angiography (PTCA) reduces progression to restenosis and increases event-free survival rates. Our aim was to determine whether EX inhibits lesion development and/or alters the extracellular matrix (ECM) composition of the neointima (NI) in a porcine PTCA model. Miniature Yucatan swine were assigned to cage confinement (SED) or EX for 20 wk. After 16 wk, all animals underwent a PTCA procedure of the left anterior descending artery (LAD) and left circumflex artery (LCX), with subsequent placement of an externalized jugular catheter. Animals recovered for 2 days and then resumed the previous protocol of SED or EX. Twelve days following PTCA, all animals received an intravenous bromodeoxyuridine (BrdU) injection to label proliferating cells. At 28 days following PTCA, the animals were euthanized, the LAD and LCX excised, and underwent standard histological processing for total collagen, type I collagen, fibronectin, BrdU, and Verhoeff-van Gieson stain. Our results demonstrate that EX significantly decreased lesion size and NI proliferation (−48%) in the LAD ( P < 0.05) but not the LCX. Furthermore, EX attenuated type I collagen expression only in LAD, whereas total collagen was increased (5.9%) and fibronectin was decreased (−7.9%) in the NI of both vessels ( P < 0.05). In conclusion, EX following PTCA may increase event-free survival rates following PTCA by decreasing lesion size and altering ECM composition.Keywords
This publication has 43 references indexed in Scilit:
- Absence of regulated splicing of fibronectin EDA exon reduces atherosclerosis in miceAtherosclerosis, 2008
- Inhibition of Intrinsic Interferon-γ Function Prevents Neointima Formation After Balloon InjuryHypertension, 2007
- Neutralization of Interleukin-18 Inhibits Neointimal Formation in a Rat Model of Vascular InjuryCirculation, 2006
- Type I collagen synergistically enhances PDGF-induced smooth muscle cell proliferation through pp60src-dependent crosstalk between the α2β1 integrin and PDGFβ receptorBiochemical and Biophysical Research Communications, 2004
- Deletion of the alternatively spliced fibronectin EIIIA domain in mice reduces atherosclerosisBlood, 2004
- Fibronectin Promotes Cell Cycle Entry in Smooth Muscle Cells in Primary CultureExperimental Cell Research, 2002
- Enhanced L‐type Ca2+ channel current density in coronary smooth muscle of exercise‐trained pigs is compensated to limit myoplasmic free Ca2+ accumulationThe Journal of Physiology, 2000
- The Extracellular Matrix Dynamically Regulates Smooth Muscle Cell Responsiveness to PDGFaAnnals of the New York Academy of Sciences, 2000
- Balloon Catheterization Induces Arterial Expression of Embryonic FibronectinsArteriosclerosis, Thrombosis, and Vascular Biology, 1995
- Expression of extra domain A fibronectin sequence in vascular smooth muscle cells is phenotype dependent.Journal of Cell Biology, 1989