In Vitro Development of Human Killer–Immunoglobulin Receptor-Positive NK Cells
- 9 November 2009
- book chapter
- Published by Springer Nature
- Vol. 612, 15-26
Abstract
The in vitro culture system outlined in this chapter allows for the delineation of events that occur during the development of CD34+ hematopoietic precursor cells into mature KIR+ human NK cells. This system can also be utilized to study the effects of gene overexpression or knockdown on the process of NK cell differentiation through retroviral transduction and long-term culture. The necessary soluble factors and contact-dependent conditions for in vitro human NK cell development have been worked out in our laboratory over the past 16 years.Keywords
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