The Unfolded Protein Response
Authors:
- 17 October 2017
- book chapter
- Published by Springer Nature
- Vol. 1691, 223-230
Abstract
Under the unfolded protein response (UPR), transcripts encoding the endoplasmic reticulum (ER) chaperones are increased and those encoding proteins synthesized in the ER are decreased. To reproducibly detect such changes of an expression profile, homogeneous growth of plants is desired. In addition, uniform treatment with drugs inducing the UPR is also necessary. Here we describe our methods of plant culture and drug treatment, and procedure to detect gene expression by quantitative RT-PCR.Keywords
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