Developing Microelectrode Arrays for the Point-of-Care Multiplex Detection of Metabolites

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Abstract
DNA-aptamer-functionalized electrode arrays can provide an intriguing method for detecting pathogen-derived exometabolites. This work addresses the limitations of previous aptamer-based pathogen detection methods by introducing a novel surface design that bridges the gap between initial efforts in this area and the demands of a point-of-care device. Specifically, the use of a diblock copolymer coating on a high-density microelectrode array and Cu-mediated cross coupling reactions that allow for the exclusive functionalization of that coating by any electrode or set of electrodes in the array provides a device that is stable for 1 year and compatible with the multiplex detection of small-molecule targets. The new chemistry developed allows one to take advantage of a large number of electrodes in the array with one experiment described herein capitalizing on the use of 960 individually addressable electrodes.
Funding Information
  • NSF Center for Synthetic Organic Electrochemistry, University of Utah (CHE-2002158)
  • National Institute of General Medical Sciences (1R01 GM122747, R35 GM144075)

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