Polystyrene nanoplastics induce apoptosis of human kidney proximal tubular epithelial cells via oxidative stress and MAPK signaling pathways
- 4 October 2023
- journal article
- research article
- Published by Springer Nature in Environmental Science and Pollution Research
- Vol. 30 (51) , 110579-110589
Abstract
S Polystyrene nanoplastics (PS-NPs) have recently been found to be present in human blood and kidney. However, the renal toxicity of PS-NPs and the underlying mechanisms have not been fully elucidated. Here, we found that exposure of PS-NPs induced apoptosis of human renal proximal tubular epithelial cells (HK-2) in a size- and dose-dependent manner as revealed by AnnexinV-FITC assay. In addition, PS-NPs promoted ROS production and caused structure changes of mitochondrial and endoplasmic reticulum. Mechanistically, transcriptional sequencing indicated the involvement of MAPK pathway in apoptosis, which was further confirmed by the upregulation of p-p38, p-ERK, CHOP, BAX, cytochrome C, and caspase 3 expression. This study clarified the molecular mechanism underlying PS-NP-induced apoptosis in HK-2 cells and contributed to our risk estimation of PS-NPs in human kidney.Keywords
Funding Information
- National Natural Science Foundation of China (82170523, 81870261)
- Sichuan Province Youth Science and Technology Innovation Team (2020JDTD0024)
- Sichuan Provincial Science and Technology Support Program (2022YFS0610)
- Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Southwest Medical University (xtcx2016-14, xtcx 2019-06)
This publication has 39 references indexed in Scilit:
- Plastic waste inputs from land into the oceanScience, 2015
- Cationic polystyrene nanospheres induce autophagic cell death through the induction of endoplasmic reticulum stressNanoscale, 2014
- Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2α/ATF4 axis in selenite-treated NB4 cellsCell Death & Disease, 2014
- Functional Roles of p38 Mitogen-Activated Protein Kinase in Macrophage-Mediated Inflammatory ResponsesMediators of Inflammation, 2014
- Pivotal role for ROS activation of p38 MAPK in the control of differentiation and tumor-initiating capacity of glioma-initiating cellsStem Cell Research, 2014
- Mitochondrial ROS production and subsequent ERK phosphorylation are necessary for temperature preconditioning of isolated ventricular myocytesCell Death & Disease, 2012
- Activation of Bak and Bax through c-Abl-Protein Kinase Cδ-p38 MAPK Signaling in Response to Ionizing Radiation in Human Non-small Cell Lung Cancer CellsJournal of Biological Chemistry, 2006
- Acute toxicity of nano- and micro-scale zinc powder in healthy adult miceToxicology Letters, 2006
- p38 Mitogen-Activated Protein Kinase (MAPK) Is a Key Mediator in Glucocorticoid-Induced Apoptosis of Lymphoid Cells: Correlation between p38 MAPK Activation and Site-Specific Phosphorylation of the Human Glucocorticoid Receptor at Serine 211Molecular Endocrinology, 2005
- The First α Helix of Bax Plays a Necessary Role in Its Ligand-Induced Activation by the BH3-Only Proteins Bid and PUMAMolecular Cell, 2004