Novel subunit—subunit interactions in the structure of glutamine synthetase
Authors:
- Robert J. AlmassyRARobert J. Almassy
- Cheryl A. JansonCJCheryl A. Janson
- R. HamlinRHR. Hamlin
- N-H. XuongNXN-H. Xuong
- David EisenbergDEDavid Eisenberg
- 1 September 1986
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 323 (6086) , 304-309
Abstract
We present an atomic model for glutamine synthetase, an enzyme of central importance in bacterial nitrogen metabolism, from X-ray crystallography. The 12 identical subunits are arranged as the carbon atoms in two face-to-face benzene rings, with unusual subunit contacts. Our model, which places the active sites at the subunit interfaces, suggests a mechanism for the main functional role of glutamine synthetase: how the enzyme regulates the rate of synthesis of glutamine in response to covalent modification and feedback inhibition.Keywords
This publication has 46 references indexed in Scilit:
- Isolation and crystallization of unadenylylated glutamine synthetase from Salmonella typhimuriumArchives of Biochemistry and Biophysics, 1984
- Formation and structure of 1-, 3- and 6+1-stranded helical cables of glutamine synthetaseInternational Journal of Biological Macromolecules, 1984
- Characteristics of a flat multiwire area detector for protein crystallographyJournal of Applied Crystallography, 1981
- Localization of the site of adenylylation of glutamine synthetase by electron microscopy of an enzyme-antibody complexProceedings of the National Academy of Sciences, 1978
- New crystal forms of glutamine synthetase and implications for the molecular structureJournal of Molecular Biology, 1978
- Packing in a new crystalline form of glutamine synthetase from Escherichia coliJournal of Molecular Biology, 1976
- Glutamine synthetase forms three- and seven-stranded helical cables.Proceedings of the National Academy of Sciences, 1975
- 24. The Glutamine Synthetase of Escherichia coli: Structure and Control1974
- Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coliBiochemistry, 1968
- Regulation of glutamine synthetase. VII. Adenylyl glutamine synthetase: a new form of the enzyme with altered regulatory and kinetic properties.Proceedings of the National Academy of Sciences, 1967