Recombination between snowhoe hare and La Crosse bunyaviruses

Authors:
  • JGJ R Gentsch
    J R Gentsch
  • GRG Robeson
    G Robeson
  • DBD H Bishop
    D H Bishop
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Abstract
Heterologous genetic recombination was reported for crosses involving temperature-sensitive (ts) mutants of La Crosse (LAC) group II and snowshoe hare (SSH) group I ts mutants. From those crosses 2 reassortant viruses having the large/medium/small viral RNA segment genotypes of SSH/LAC/SSH and SSH/LAC/LAC were obtained. The reciprocal cross (SSH group II .times. LAC group I ts mutants) did not yield the expected LAC/SSH/SSH or LAC/SSH/LAC reassortant viruses. The backcross of a SSH/LAC/SSH group II ts mutant with a LAC group I ts mutant produced a new reassortant virus, LAC/LAC/SSH, whereas the backcross of SSH/LAC/LAC group I ts mutants with SSH group II ts mutants gave another reassortant, SSH/SSH/LAC. Backcross analyses of LAC/LAC/SSH group I ts mutants with group II ts mutants of SSH did not yield the expected LAC/SSH/SSH reassortant virus, nor did backcrosses of SSH/SSH/LAC group II ts mutants with group I ts mutants of LAC virus yield the expected LAC/SSH/LAC reassortant. Possible reasons why certain reassortant viruses are not produced are discussed. A procedure to screen SSH-LAC reassortant viruses which differ in their virion N polypeptides is described.

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