Identification of a Chromosome-Borne Expanded-Spectrum Class A β-Lactamase from Erwinia persicina
Open Access
- 1 November 2002
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 46 (11) , 3401-3405
Abstract
From whole-cell DNA of an enterobacterial Erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a β-lactamase gene was cloned and expressed in Escherichia coli. It encoded a clavulanic-acid-inhibited Ambler class A β-lactamase, ERP-1, with a pI value of 8.1 and a relative molecular mass of ca. 28 kDa. ERP-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from Citrobacter koseri , Kluyvera ascorbata , Kluyvera cryocrescens , Klebsiella oxytoca , Proteus vulgaris , Proteus penneri , Rahnella aquatilis , Serratia fonticola , Yersinia enterocolitica , and the plasmid-mediated enzymes CTX-M-8 and CTX-M-9. The substrate profile of the noninducible ERP-1 was similar to that of these β-lactamases. ERP-1 is the first extended-spectrum β-lactamase from an enterobacterial species that is plant associated and plant pathogenic.Keywords
This publication has 30 references indexed in Scilit:
- Characterization of a Chromosomally Encoded Extended-Spectrum Class A β-Lactamase from Kluyvera cryocrescensAntimicrobial Agents and Chemotherapy, 2001
- Biochemical-Genetic Characterization of the Chromosomally Encoded Extended-Spectrum Class A β-Lactamase from Rahnella aquatilisAntimicrobial Agents and Chemotherapy, 2001
- Erwinia chrysanthemistrains cause death of human gastrointestinal cells in culture and express an intimin-like proteinFEMS Microbiology Letters, 2000
- Crystal structure of the E166A mutant of extended-spectrum β-lactamase toho-1 at 1.8 Å resolution 1 1Edited by R. HuberJournal of Molecular Biology, 1999
- Bacterial production of carbapenems and clavams: evolution of β-lactam antibiotic pathwaysTrends in Microbiology, 1998
- Characterization and amino acid sequence analysis of a new oxyimino cephalosporin-hydrolyzing class A β-lactamase from Serratia fonticola CUVBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1997
- Inducible Expression of the Chromosomal cdiA from Citrobacter diversus NF85, Encoding an Ambler Class A β-Lactamase, Is under Similar Genetic Control to the Chromosomal ampC , Encoding an Ambler Class C Enzyme, from Citrobacter freundii OS60Microbial Drug Resistance, 1995
- Chromosomally encoded cephalosporin-hydrolyzing β-lactamase of Proteus vulgaris RO104 belongs to Ambler's class ABiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1994
- Cloning and nucleotide sequencing of the gene encoding the β-lactamase from Citrobacter diversusFEMS Microbiology Letters, 1991
- Erwinia persicinus, a New Species Isolated from PlantsInternational Journal of Systematic and Evolutionary Microbiology, 1990