Saccharomyces cerevisiae Pex14p contains two independent Pex5p binding sites, which are both essential for PTS1 protein import

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Abstract
Pex14p is a peroxisomal membrane‐associated protein involved in docking of both Pex5p and Pex7p to the peroxisomal membrane. Previous studies have shown that, in humans, the N‐terminal region of Pex14p interacts with WxxxF/Y motifs in Pex5p. Here, we report that Saccharomyces cerevisiae Pex14p contains two independent Pex5p binding sites, one in the N‐ and one in the C‐terminus. Using deletion analysis we show that, in vivo, both of these interactions are needed for PTS1 import. Furthermore, we show that the characterized WxxxF/Y motifs of Pex5p are not essential for binding to the N‐terminus of Pex14p but do play a role in the interaction with the Pex14 C‐terminus. Thus, the data suggest that the mechanism of the Pex14p–Pex5p interaction in yeast is different from that previously reported for humans.

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