Ultrasensitive Immunosensor for Cardiac Troponin I Detection Based on the Electrochemiluminescence of 2D Ru-MOF Nanosheets

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Abstract
Electrochemiluminescence (ECL)-functionalized metal-organic frameworks (MOFs) have attracted increasing attention in target sensing in virtue of its diverse and tunable optical properties. A famous ECL luminophore, carboxyl-rich tris(4,4′-dicarboxylicacid-2,2′-bipyridyl) ruthenium(II) (Ru(dcbpy)32+) possesses the characteristics of good water solubility and excellent ECL performance, which also has the potential to be organic ligand of metal-organic frameworks. Herein, functionalized MOF nanosheets (RuMOFNSs) containing plenty of Ru(dcbpy)32+ in the frameworks was synthesized in aqueous solution by simple one-pot method. In this protocol, Ru(dcbpy)32+ acted as organic ligand to coordinate with Zn2+ originated from Zn(NO3)2, polyvinylpyrrolidone (PVP) was used as structure-directing agent to control the formation of sheet-like structure. For the practical application, a “signal-on” ECL immunosensor was designed for cardiac troponin I (cTnI) detection by employing RuMOFNSs as ECL probe. The immunosensor exhibited high sensitivity and excellent selectivity for cTnI detection in the range from 1 fg/mL to 10 ng/mL with a low detection limit as low as 0.48 fg/mL. Finally, the biosensor was successfully applied for the detection of cTnI in human serum sample with satisfactory results, demonstrating its potential application in bioanalysis and clinical diagnosis.
Funding Information
  • Ministry of Science and Technology of the People's Republic of China (2016YFA0201301)
  • People's Government of Jilin Province (20170101195JC)
  • National Natural Science Foundation of China (21435005, 21627808)
  • Chinese Academy of Sciences (QYZDY-SSW-SLH019)

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