High performance size exclusion chromatography of fatty acids, mono‐, di‐ and triglyceride mixtures

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Abstract
A high performance size exclusion chromatographic (HPSEC) method is described for the separation and quantitation of fatty acids, mono‐, di‐ and triglyceride mixtures. The various lipid components were separated on two columns packed with 5 µm styrene/divinylbenzene copolymer and connected in series. Toluene was employed as eluant, and components were monitored by refractometry. A formula derived for calculation of total weighted correction factors (WCF) for the various lipid classes based on known values of correction factors of simple lipid components and the fatty acid composition of the sample allowed quantitation of lipid mixtures containing a variety of different molecules. The precision of the experiments is such that the relative standard deviation for each lipid component was 1–5%, and a component could be detected at 0.05% level.

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